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hmsc osteogenic differentiation basal medium  (Lonza)
90
Lonza hmsc osteogenic differentiation basal medium
Effects of EGCG-loaded HA on coculture of <t>hMSC-derived</t> osteoblasts and monocyte derived osteoclasts were evaluated by SEM microscopy and relative gene expression.35 (A) SEM micrographs showed healthy osteoblast morphology in all samples at all time points. The control showed healthy maturation from differentiated monocytes (white circle) pre-osteoclast cells. The osteoclast cells bonded to form multi-nucleated mature osteoclasts at days 7 and 16. EGCG showed good adhesion and attachment of osteoclast cells, but inhibited maturation compared to the control at that time. (B)–(E) qRT-PCR results were presented as fold change compared to the control, which is standardized to a value of 1. GAPDH was used to normalize all gene expression data. Day 7 was the early time point to observe the effects of EGCG on coculture. The higher expression of (B) bone gamma-carboxyglutamic acid-containing protein (BGLAP) and (C) Runt-related transcription factor 2 (Runx2) in treatment than control implied that EGCG stimulated osteoblast differentiation and maturation on day 16. However, EGCG inhibited osteoclast differentiation and activity by upregulating (D) osteoprotegerin (OPG), a negative regulator of osteoclastogenesis, and downregulating (E) RANKL expression on day 16. (* denotes p value ≤ 0.05, ** denotes p value ≤ 0.001).
Hmsc Osteogenic Differentiation Basal Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hmsc+differentiation+basal+medium/human+msc+osteogenic+differentiation+medium/pmc11132590-60-11-16
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hmsc osteogenic differentiation basal medium - by Bioz Stars, 2026-10
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hmsc osteogenic differentiation basal medium pt-3924  (Lonza)
90
Lonza hmsc osteogenic differentiation basal medium pt-3924
Effects of EGCG-loaded HA on coculture of <t>hMSC-derived</t> osteoblasts and monocyte derived osteoclasts were evaluated by SEM microscopy and relative gene expression.35 (A) SEM micrographs showed healthy osteoblast morphology in all samples at all time points. The control showed healthy maturation from differentiated monocytes (white circle) pre-osteoclast cells. The osteoclast cells bonded to form multi-nucleated mature osteoclasts at days 7 and 16. EGCG showed good adhesion and attachment of osteoclast cells, but inhibited maturation compared to the control at that time. (B)–(E) qRT-PCR results were presented as fold change compared to the control, which is standardized to a value of 1. GAPDH was used to normalize all gene expression data. Day 7 was the early time point to observe the effects of EGCG on coculture. The higher expression of (B) bone gamma-carboxyglutamic acid-containing protein (BGLAP) and (C) Runt-related transcription factor 2 (Runx2) in treatment than control implied that EGCG stimulated osteoblast differentiation and maturation on day 16. However, EGCG inhibited osteoclast differentiation and activity by upregulating (D) osteoprotegerin (OPG), a negative regulator of osteoclastogenesis, and downregulating (E) RANKL expression on day 16. (* denotes p value ≤ 0.05, ** denotes p value ≤ 0.001).
Hmsc Osteogenic Differentiation Basal Medium Pt 3924, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hmsc+differentiation+basal+medium/human+msc+osteogenic+differentiation+medium/pmc10720363-131-24-30
Average 90 stars, based on 1 article reviews
hmsc osteogenic differentiation basal medium pt-3924 - by Bioz Stars, 2026-10
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hmsc differentiation basal medium-osteogenic  (Lonza)
90
Lonza hmsc differentiation basal medium-osteogenic
Effects of EGCG-loaded HA on coculture of <t>hMSC-derived</t> osteoblasts and monocyte derived osteoclasts were evaluated by SEM microscopy and relative gene expression.35 (A) SEM micrographs showed healthy osteoblast morphology in all samples at all time points. The control showed healthy maturation from differentiated monocytes (white circle) pre-osteoclast cells. The osteoclast cells bonded to form multi-nucleated mature osteoclasts at days 7 and 16. EGCG showed good adhesion and attachment of osteoclast cells, but inhibited maturation compared to the control at that time. (B)–(E) qRT-PCR results were presented as fold change compared to the control, which is standardized to a value of 1. GAPDH was used to normalize all gene expression data. Day 7 was the early time point to observe the effects of EGCG on coculture. The higher expression of (B) bone gamma-carboxyglutamic acid-containing protein (BGLAP) and (C) Runt-related transcription factor 2 (Runx2) in treatment than control implied that EGCG stimulated osteoblast differentiation and maturation on day 16. However, EGCG inhibited osteoclast differentiation and activity by upregulating (D) osteoprotegerin (OPG), a negative regulator of osteoclastogenesis, and downregulating (E) RANKL expression on day 16. (* denotes p value ≤ 0.05, ** denotes p value ≤ 0.001).
Hmsc Differentiation Basal Medium Osteogenic, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hmsc+differentiation+basal+medium/human+msc+osteogenic+differentiation+medium/pmc09345981-289-9-13
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hmsc differentiation basal medium-osteogenic - by Bioz Stars, 2026-10
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human mesenchymal stem cell (hmsc) chondrogenic differentiation (basal) medium  (Lonza)
90
Lonza human mesenchymal stem cell (hmsc) chondrogenic differentiation (basal) medium
Alcian blue, type I/II/X collagen, and DAPI staining for <t>chondrogenic</t> pellets. 250000 MPCs were pelleted in each condition on day 0. The control condition contained chondrogenic basal medium without growth factor supplementation (negative control). The control + TGF-β3 condition contained control medium with the addition of 10ng/mL TGF-β3 (positive control). Static (Stat) and SSB (Dyn) conditions contained negative control medium with the addition of EVs from static and SSB (80rpm) culture conditions. Pellets were fixed after 30 days of culture, sectioned, and stained accordingly. Pellet quality scoring was identified by quantifying the MFI of each channel ( n = 6). Scale bars = 25 µM. Abbreviations: DAPI, 4ʹ,6-diamidino-2-phenylindole; EVs, extracellular vesicles; MFI, mean fluorescent intensity; MPCs, <t>mesenchymal</t> progenitor cells; SSB, stirred suspension bioreactor; TGF, transforming growth factor.
Human Mesenchymal Stem Cell (Hmsc) Chondrogenic Differentiation (Basal) Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hmsc+differentiation+basal+medium/human+mscs/pmc08895489-115-0-9
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human mesenchymal stem cell (hmsc) chondrogenic differentiation (basal) medium - by Bioz Stars, 2026-10
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hmsc osteogenic differentiation medium  (Lonza)
95
Lonza hmsc osteogenic differentiation medium
Alcian blue, type I/II/X collagen, and DAPI staining for <t>chondrogenic</t> pellets. 250000 MPCs were pelleted in each condition on day 0. The control condition contained chondrogenic basal medium without growth factor supplementation (negative control). The control + TGF-β3 condition contained control medium with the addition of 10ng/mL TGF-β3 (positive control). Static (Stat) and SSB (Dyn) conditions contained negative control medium with the addition of EVs from static and SSB (80rpm) culture conditions. Pellets were fixed after 30 days of culture, sectioned, and stained accordingly. Pellet quality scoring was identified by quantifying the MFI of each channel ( n = 6). Scale bars = 25 µM. Abbreviations: DAPI, 4ʹ,6-diamidino-2-phenylindole; EVs, extracellular vesicles; MFI, mean fluorescent intensity; MPCs, <t>mesenchymal</t> progenitor cells; SSB, stirred suspension bioreactor; TGF, transforming growth factor.
Hmsc Osteogenic Differentiation Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hmsc+differentiation+basal+medium/hMSC+-+Human+Mesenchymal+Stem+Cell+Osteogenic+Differentiation+Basal+Medium/pmc07391263-135-19-23
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hmsc osteogenic differentiation medium - by Bioz Stars, 2026-10
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hmsc differentiation basal medium–osteogenic with singlequots  (Lonza)
90
Lonza hmsc differentiation basal medium–osteogenic with singlequots
Alcian blue, type I/II/X collagen, and DAPI staining for <t>chondrogenic</t> pellets. 250000 MPCs were pelleted in each condition on day 0. The control condition contained chondrogenic basal medium without growth factor supplementation (negative control). The control + TGF-β3 condition contained control medium with the addition of 10ng/mL TGF-β3 (positive control). Static (Stat) and SSB (Dyn) conditions contained negative control medium with the addition of EVs from static and SSB (80rpm) culture conditions. Pellets were fixed after 30 days of culture, sectioned, and stained accordingly. Pellet quality scoring was identified by quantifying the MFI of each channel ( n = 6). Scale bars = 25 µM. Abbreviations: DAPI, 4ʹ,6-diamidino-2-phenylindole; EVs, extracellular vesicles; MFI, mean fluorescent intensity; MPCs, <t>mesenchymal</t> progenitor cells; SSB, stirred suspension bioreactor; TGF, transforming growth factor.
Hmsc Differentiation Basal Medium–Osteogenic With Singlequots, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hmsc+differentiation+basal+medium/human+msc+osteogenic+differentiation+medium/pmc07312587-210-27-40
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hmsc differentiation basal medium–osteogenic with singlequots - by Bioz Stars, 2026-10
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hmsc osteogenic differentiation basal medium  (Cyagen Biosciences)
90
Cyagen Biosciences hmsc osteogenic differentiation basal medium
Alcian blue, type I/II/X collagen, and DAPI staining for <t>chondrogenic</t> pellets. 250000 MPCs were pelleted in each condition on day 0. The control condition contained chondrogenic basal medium without growth factor supplementation (negative control). The control + TGF-β3 condition contained control medium with the addition of 10ng/mL TGF-β3 (positive control). Static (Stat) and SSB (Dyn) conditions contained negative control medium with the addition of EVs from static and SSB (80rpm) culture conditions. Pellets were fixed after 30 days of culture, sectioned, and stained accordingly. Pellet quality scoring was identified by quantifying the MFI of each channel ( n = 6). Scale bars = 25 µM. Abbreviations: DAPI, 4ʹ,6-diamidino-2-phenylindole; EVs, extracellular vesicles; MFI, mean fluorescent intensity; MPCs, <t>mesenchymal</t> progenitor cells; SSB, stirred suspension bioreactor; TGF, transforming growth factor.
Hmsc Osteogenic Differentiation Basal Medium, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hmsc+differentiation+basal+medium/hmsc+osteogenic+differentiation+basal+medium/pm30277597-40-7-34
Average 90 stars, based on 1 article reviews
hmsc osteogenic differentiation basal medium - by Bioz Stars, 2026-10
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Effects of EGCG-loaded HA on coculture of hMSC-derived osteoblasts and monocyte derived osteoclasts were evaluated by SEM microscopy and relative gene expression.35 (A) SEM micrographs showed healthy osteoblast morphology in all samples at all time points. The control showed healthy maturation from differentiated monocytes (white circle) pre-osteoclast cells. The osteoclast cells bonded to form multi-nucleated mature osteoclasts at days 7 and 16. EGCG showed good adhesion and attachment of osteoclast cells, but inhibited maturation compared to the control at that time. (B)–(E) qRT-PCR results were presented as fold change compared to the control, which is standardized to a value of 1. GAPDH was used to normalize all gene expression data. Day 7 was the early time point to observe the effects of EGCG on coculture. The higher expression of (B) bone gamma-carboxyglutamic acid-containing protein (BGLAP) and (C) Runt-related transcription factor 2 (Runx2) in treatment than control implied that EGCG stimulated osteoblast differentiation and maturation on day 16. However, EGCG inhibited osteoclast differentiation and activity by upregulating (D) osteoprotegerin (OPG), a negative regulator of osteoclastogenesis, and downregulating (E) RANKL expression on day 16. (* denotes p value ≤ 0.05, ** denotes p value ≤ 0.001).

Journal: Journal of materials chemistry. B

Article Title: In vitro biological evaluation of epigallocatechin gallate (EGCG) release from three-dimensional printed (3DP) calcium phosphate bone scaffolds

doi: 10.1039/d2tb02210a

Figure Lengend Snippet: Effects of EGCG-loaded HA on coculture of hMSC-derived osteoblasts and monocyte derived osteoclasts were evaluated by SEM microscopy and relative gene expression.35 (A) SEM micrographs showed healthy osteoblast morphology in all samples at all time points. The control showed healthy maturation from differentiated monocytes (white circle) pre-osteoclast cells. The osteoclast cells bonded to form multi-nucleated mature osteoclasts at days 7 and 16. EGCG showed good adhesion and attachment of osteoclast cells, but inhibited maturation compared to the control at that time. (B)–(E) qRT-PCR results were presented as fold change compared to the control, which is standardized to a value of 1. GAPDH was used to normalize all gene expression data. Day 7 was the early time point to observe the effects of EGCG on coculture. The higher expression of (B) bone gamma-carboxyglutamic acid-containing protein (BGLAP) and (C) Runt-related transcription factor 2 (Runx2) in treatment than control implied that EGCG stimulated osteoblast differentiation and maturation on day 16. However, EGCG inhibited osteoclast differentiation and activity by upregulating (D) osteoprotegerin (OPG), a negative regulator of osteoclastogenesis, and downregulating (E) RANKL expression on day 16. (* denotes p value ≤ 0.05, ** denotes p value ≤ 0.001).

Article Snippet: Human mesenchymal stem cell (hMSC) osteogenic differentiation media was prepared using hMSC osteogenic differentiation basal medium (Lonza, USA), and hMSC osteogenic differentiation singlequotsTM supplements kit (Lonza, USA).

Techniques: Derivative Assay, Microscopy, Gene Expression, Control, Quantitative RT-PCR, Expressing, Activity Assay

Alcian blue, type I/II/X collagen, and DAPI staining for chondrogenic pellets. 250000 MPCs were pelleted in each condition on day 0. The control condition contained chondrogenic basal medium without growth factor supplementation (negative control). The control + TGF-β3 condition contained control medium with the addition of 10ng/mL TGF-β3 (positive control). Static (Stat) and SSB (Dyn) conditions contained negative control medium with the addition of EVs from static and SSB (80rpm) culture conditions. Pellets were fixed after 30 days of culture, sectioned, and stained accordingly. Pellet quality scoring was identified by quantifying the MFI of each channel ( n = 6). Scale bars = 25 µM. Abbreviations: DAPI, 4ʹ,6-diamidino-2-phenylindole; EVs, extracellular vesicles; MFI, mean fluorescent intensity; MPCs, mesenchymal progenitor cells; SSB, stirred suspension bioreactor; TGF, transforming growth factor.

Journal: Stem Cells Translational Medicine

Article Title: Production of Mesenchymal Progenitor Cell-Derived Extracellular Vesicles in Suspension Bioreactors for Use in Articular Cartilage Repair

doi: 10.1093/stcltm/szab008

Figure Lengend Snippet: Alcian blue, type I/II/X collagen, and DAPI staining for chondrogenic pellets. 250000 MPCs were pelleted in each condition on day 0. The control condition contained chondrogenic basal medium without growth factor supplementation (negative control). The control + TGF-β3 condition contained control medium with the addition of 10ng/mL TGF-β3 (positive control). Static (Stat) and SSB (Dyn) conditions contained negative control medium with the addition of EVs from static and SSB (80rpm) culture conditions. Pellets were fixed after 30 days of culture, sectioned, and stained accordingly. Pellet quality scoring was identified by quantifying the MFI of each channel ( n = 6). Scale bars = 25 µM. Abbreviations: DAPI, 4ʹ,6-diamidino-2-phenylindole; EVs, extracellular vesicles; MFI, mean fluorescent intensity; MPCs, mesenchymal progenitor cells; SSB, stirred suspension bioreactor; TGF, transforming growth factor.

Article Snippet: Human Mesenchymal Stem Cell (hMSC) Chondrogenic Differentiation (basal) Medium (Lonza, Basel, Switzerland) was used as a negative control.

Techniques: Staining, Control, Negative Control, Positive Control, Suspension